Oh, and keep an eye out next Monday – I’m sharing my favorite tool for finding and deciding on a passion-based career! But have you ever noticed that the busiest and most accomplished people never seem to say them? It’s no wonder why much of the world spins their tires on email, Facebook, Twitter and petty tasks and requests, complaining about hating their work and dreaming of a new career, while the Living Legends change the world. The simple purpose is to connect your dreams, outcomes, and desires to the actions you take on a daily and weekly basis. Remember, there’s a free workbook download of the full process at the bottom of this article for you to actually fill out each week. Just be sure you do it before you start any of your weekly tasks, and especially before you check email! Do This: Look back through your notes and ideas from last week and list all the lessons that come to mind.
Decide on on a maximum of 6 to 7 outcomes you want to accomplish related to the various areas in your life. One of my big outcomes for this week is: writing this article and the free workbook to go with it (you can download that at the bottom).
Look at your 6 to 7 weekly outcomes and decide what core tasks will need to happen to accomplish these.
There is a reason why the people who get the most done tend to continue to be able to do more and more of what matters. If you are not a subscriber yet, entering your email below will give you immediate access to our full suite of free tools and you’ll get an update every time a new article comes out, as well as some other exclusive subscriber-only content.
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Description: This podcast covers several new topics, including celebrities who are convinced the earth is flat, the growing threat that is Zika virus, and how HIV is eluding CRISPR treatments. Keywords: glycans, glycobiology, podcast, Pascal Gagneux, sperm, evolution, biolegend, science, chimpanzees, non-human primates, Neanderthal, pathogen, Ajit Varki Having difficulty playing this episode?
Description: In this episode, we discuss some a variety of current scientific news, including the discovery of woman who can smell Parkinson's disease, a correlation between bovine leukemia virus and breast cancer, using an individual's microbial cloud to identify them, and how we're using specialized antibodies to combat both HIV and cancer.
Keywords: immunology, comics, cancer, chimeric antigen receptor, T cells, gene therapy, hypoxia, immune checkpoints, goinvivo, tumors, podcast, pedromics, pedro velica Having difficulty playing this episode? Keywords: neuroscience, neurodegeneration, Parkinson's disease, microbes, microbial, gut microbiome, bacteria, asthma, allergy, leukemia, cancer, AML, antibody, virus, cow, bovine, breast cancer, HIV, AIDS Having difficulty playing this episode? Description: Special guest and global health scientist Jessica Taaffe joins us to discuss educating the public, funding research, and the passion needed in scientific role models. Keywords: global health, Jessica Taaffe, HIV, ebola, education, Bill Nye, Neil degrasse Tyson, vaccines, anti-vaxxers, world bank, funding, research, NIH, primates, biolegend, podcast Having difficulty playing this episode?
Description: The podcast team discusses the potential anti-venom shortage, a wasp-venom derived protein for cancer therapy, 30,000 year-old viruses, and a new hominid species discovered in South Africa. Keywords: venom, anti-venom, snakebites, wasp venom, Fav-Afrique, MP1, Polybia-MP1, Polybia paulista, phosphatidylserine, annexin V, cancer cells, homo naledi, fossils, Mollivirus sibericum, viruses, Siberia, giant viruses Having difficulty playing this episode? Description: On this podcast, we discuss current events in science, including recent outbreaks of polio in the Ukraine and Legionnaire's disease in California and Illinois. Keywords: polio, Legionnaire's disease, outbreaks, virus, bacteria, saturated fats, obesity, microbiome, mice, inflammation, leptin, neurons, brain, exercise, neutrophils, T cells, infection, lungs, influenza, open-access, journal, the wall of polio, science, podcast, virology, immunology Having difficulty playing this episode?
Description: Glioblastomas and medulloblastomas are two common forms of brain cancer that afflicts thousands of people a year. Description: We cover the latest science current events, including the new Ebola vaccine, limb regeneration, being hangry, and Kim K's flub with the FDA. Keywords: Kim Kardashian, ebola, vaccines, anti-vaxxers, current events, limb regeneration, repair, hangry, science, podcast, food and drug administration, morning sickness, FDA Having difficulty playing this episode?
Description: In our latest podcast, we talk about narcolepsy and the fight between local colleges for the rights to an Alzheimer's disease database.
Keywords: UCSD, USC, University of Southern California, University of California San Diego, Alzheimer's disease, Pandemrix, narcolepsy, sleep, Paul Aisen, BioLegend, science, podcast Having difficulty playing this episode?
Description: Ed Chen and Rea Dabelic, two members of the BioLegend Technical Team, take over the podcast this week to discuss Selman Waksman (the Father of Antibiotics), tuberculosis, and giving credit where its due.
Keywords: Selman Waksman, Albert Schatz, streptomycin, neomycin, antibiotics, Neosporin, tuberculosis, granulomas, macrophage, biolegend, immunology, mycobacterium tuberculosis, podcast, patents, inventions, royalties, tuberculin skin test, lab assistants, consumption, avastin, microbiology, birthday blog, controversy, Nobel prize Having difficulty playing this episode? Description: We welcome Chris Gould to discuss neuroscience, protein misfolding, aggregation, and prion-like proteins in neurodegenerative diseases. Keywords: neuroscience, tau, a-synuclein, alzheimer's disease, parkinson's disease, neuroinflammation, prions, Creutzfeldt-Jakob disease, mad cow disease, kuru, cannibalism, brain, aggregation, protein misfolding, blood brain barrier, lymphatic system, chris gould, biolegend, immunology, podcast, neurofibrillary tangles, lewy bodies, inclusion, Lou Gehrig's disease, ALS, amyotrophic lateral sclerosis, neurodegeneration, microglia Having difficulty playing this episode? Description: Neuroinflammation can be beneficial in some instances, but too much of it can cause any number of neurological diseases. Keywords: Kenya Cohane, neuroinflammation, neuroscience, alzheimer's disease, parkinson's disease, multiple sclerosis, tau protein, a-synuclein, aggregation, immunology, podcast, biolegend Having difficulty playing this episode?
Description: Antibody therapies have become incredibly popular for the treatment of diseases, including cancer. Keywords: cancer, tumor, antibody therapy, therapeutics, immune checkpoints, PD1, PDL1, LAG3, TIM3, CD80, CD86, Galectin 9, MHC II, CTLA4, Pembrolizumab, Ipilimumab, Nivolumab, nanobody, melanoma, carcinoma, biolegend, science, podcast, merck, amgen Having difficulty playing this episode? Description: CRISPR is an amazing technology originally discovered in bacteria as a defense mechanism against invading foreign genetic material.
Description: Despite our advances in science and technology, it seems like more and more people are outright denying scientific principles and concepts like evolution and climate change.
Keywords: podcast, biolegend, immunology, biology, climate change, ted cruz, NASA, jim inholfe, brain, neuroscience, music, ignorance, science, education, ivory tower, spock, star trek, leonard nimoy, evolution, fluorine, fluoridination, fanta, Tourette Syndrome, nerdy, nerds, nerd, fandom Having difficulty playing this episode? Description: In this podcast, we discuss bacterial hot spots in New York, a brand new antibiotic found in dirt, telomeres and aging, and an interesting article published in 17 scientific journals about Cocoa Puffs. Keywords: podcast, podcasts, New York, subways, bacteria, antibiotics, MRSA, antibiotic resistance, telomeres, cocoa puffs, teixobactin, beer, alzheimer’s disease, parkinson’s disease, aging, BioLegend, immunology, biology, science, grad school, scientific journals Having difficulty playing this episode?


Description: In this podcast, we take a look at how a lack of vaccinations has once again caused an outbreak of a once-controlled disease, the efficacy of the flu vaccine this year, and why people are clamoring to know if their food contains DNA. Keywords: podcast, science, measles, vaccinations, vaccines, anti-vaxxers, Jenny McCarthy, research, cures, Disney, Disneyland, outbreak, flu vaccine, influenza, flu, dna, food, GMO, genetically modified organism, antibiotics, virus, viruses Having difficulty playing this episode? Description: In this podcast, we examine why it’s so hard to transition from animal research into human cures and Obama’s latest visit to the NIH.
Keywords: podcast, president, Obama, Barack Obama, BioLegend, NIH, National Institutes of Health, science, Ebola, HIV, Human immunodeficiency virus, AIDS, acquired immune deficiency syndrome, translational models, animal models, translation, research, bench to bedside, cures, vaccines Having difficulty playing this episode? I’ve also included a free Weekly Planning Worksheet download at the bottom, to make sure this stuff is super easy for you to do.
It’s not because they were born with something special or only sleep three hours a night. Without a process for doing what matters, on a daily and hourly basis, the odds will kill you. I find very little logic in starting from scratch these days – I prefer to build off of what already works. They’ve worked together for nearly two decades (like from before most people knew who Tony was). This could be creating a personal budget, cooking a healthy meal or having a great meeting with a mentor. These need to get done, but not at the expense of the things that help us accomplish what actually matters.
If I put it off until the end of the month, this stuff turns into more of of a mental monster than it needs to be. If you think it’s going to take an hour to write an article, then schedule an hour and a half. Given this process, front-weight your most important tasks so that no matter what comes up, at least a few of them will get done.
You have read and agree to the Global Shipping Program terms and conditions - opens in a new window or tab. Import charges previously quoted are subject to change if you increase you maximum bid amount. Contact the seller- opens in a new window or tab and request a shipping method to your location. We examine increasing cases of diabetes and how finding the "secret sauce" for beta cells might fight this trend.
BioLegend is working with Sean Parker and the Parker Institute’s collaboration in fighting back against cancer. Recent studies have reported a link between Herpes Simplex Virus 1 (HSV-1) and increased AD risk.
This blog will cover the major apps provided by BioLegend relevant to research steps from conceptualization to discovery, as well as a few useful apps not from BioLegend. Look back at this year’s most popular posts and find out which blogs our writers enjoyed composing. Our latest podcast invites guest Pascal Gagneux to discuss his thoughts on glycans, evolution, and sex cells!
Learn what makes this virus dangerous and why some countries are advising women to avoid pregnancy. Although respiration is considered the more efficient pathway because it has more ATP output per molecule of pyruvate, rapidly-dividing cells sometimes choose fermentation instead. Almost 50 BioLegend employees took part is this fun race which supports awareness for local charities trying to bring about positive change in San Diego.
By controlling key molecules involved in regulating the immune response, researchers have obtained very promising results in the treatment of certain cancers. Read this blog to learn more about Mazumdar-Shaw’s humble beginnings and how she built her empire by literally starting in her garage. Scientists would like to reanimate this virus to learn more about its origins and the evolution of giant viruses.
We also cover recent research linking high-fat diets with a change in the gut microbiome and obesity-linked inflammation, as well as the role that leptin plays in the rewarding effects of exercise. Immunotherapy is a groundbreaking option because it can hypothetically target cancer cells specifically while leaving healthy cells intact, which can decrease the number of adverse effects associated with current treatment options.
Some may notice a modest decline in their mental abilities, including memory function, while some may develop neurodegenerative diseases as they age.
Tune in to hear our guest Mohar Chattopadhyay discuss its symptoms, treatments, and research.
Using bioinformatics methods, they reconstructed the evolutionary tree of AAV and made viruses that represent different branching points. Resistance to anti-microbial drugs occurs naturally, however, the misuse of these drugs, among other factors, is helping the spread of multidrug resistant microbes. However, communication between the CNS and the periphery occurs through unknown mechanisms.
The cause of this disease is unknown, and although there is presently no cure, there are some treatment options such as medication and surgery to manage its symptoms. Learn more about neuroinflammation and the newly discovered central nervous system lymphatic system with our special guest, Kenya Cohane.
They are highly similar to peripheral macrophages and respond to pathogens and injury by becoming activated. But, how does this peer review work and why have so many controversies sprung up recently about this system?
The Neuroinflammation page shows how neuronal injury can lead to immune infiltration, and how differential activation of microglia can be either neuroprotective or neurotoxic. This versatile system can accommodate your experimental needs, whether you are doing positive or negative selection.
In cancer, it may be beneficial to prevent interaction of "immune checkpoints", which can inactivate T cells. This time we contribute to science enrichment classes in elementary schools throughout San Diego. In this blog, we discuss the potential impact of sequencing or genotyping individuals and why the FDA temporarily shutdown one company for marketing their kit as a medical device. Quite often, they manage to subvert a highly evolved immune system and proliferate inside a host. It allows researchers to listen to important talks, collaborate, and see what companies have to offer. Researchers are now repurposing this for useful applications in like increasing crop size and treating diseases like HIV and cancer. With open source sharing of 3D models, anyone with access to a 3D printer can print tube racks, 96 well plates, gel combs, and many other things. We also take a look at some of the inner workings of the brain including synchronization, implanting false memories, and how it blocks distractions. In this blog, we discuss one of the biggest scientific scandals in recent memory: The publication of high-profile stem cell papers in one of the most reputable journals that was riddled with errors, and, in at least a couple of spots, obvious data manipulation. Depending on the requirements of your assays, as well as your equipment at hand, you may need to use a colorimetric assay. This blog discusses the implications of red meat consumption in humans and the characterization Neu5Gc as a xeno-autoantigen capable of inducing low level inflammation leading to cancer.


These are called plantibodies, and in fact, there are already companies developing therapeutic antibodies using them. There we showcased some of our newly released products, such as LEGENDplex and newly aquired NeoClone antibodies.
Our blog examines possible causes, treatments, and mechanisms of action for food allergens. Keywords: BioLegend, science, podcast, podcasts, immunology, neuroscience, memory, alcohol, beer, neurons, ion channels, NMDA receptor, redox, oxidation, reduction, calcium, potassium, sodium, hippocampus, GIRK receptor, addiction, Karthik Bodhinathan, Stuart Lipton Having difficulty playing this episode? Here is a post to show some of the cool costumes and pumpkin carving contest we had this year. Tony Robbins connects and speaks with tens of thousands of people a year, all over the world, and still manages to launch new ventures non-stop. During one of our first sessions she shared Tony’s (and her) weekly planning process. At first this is as counter-intuitive as working out – but no matter how crazy your schedule is, you will be more effective, confident and calm if you take time before you jump into the storm.
Anything and everything positive goes – having a record sales week, getting a rewarding thank you, having a hot date or sticking to your workout. Write down any key learnings from the past week: major lessons, meaningful quotes and things that inspire you. You should see my Apple iCal – it looks like someone spilled a pack of Skittles on it with all the self-appointments for each big task related to my Areas of Attention. It’s totally free and I encourage you to use this as your template to actually fill out each week. If you reside in an EU member state besides UK, import VAT on this purchase is not recoverable. And, we talk about Sean Parker's new $250 million grant to establish the Parker Institute for Cancer Immunotherapy. In this blog, we discuss a landmark case study, and human trial, in which gene editing was successfully used in the treatment of leukemia. As hard as it is to secure funding, prioritizing how to stay within the lab’s budget is an equally daunting task.
The use of monoclonal antibodies against some of these molecules, called immune checkpoint receptors, have provided some answers and hope in the search for a cure. Tune in to hear our discussion on the release of an open-access journal that will publish ideas as well as data and results, and other exciting research on influenza infection. In this blog, read about adoptive cell transfer, the process of training and expanding a patient's own immune cells and placing it back into the patient to fight the cancer with their own cells. Scientists are increasingly interested to understand the factors and mechanisms that may impact brain health. These viruses were used to circumvent detection by the immune system to successfully deliver genes to various mammalian tissues. This is a serious public health issue at the moment, and if we don’t act with energy, there can be hard consequences. In this blog, we discuss a recent paper published in Nature that discovered that the CNS does, in fact, have a lymphatic system that can be used to communicate with immune cells in the periphery. Read our blog to learn more about Selman Waksman, his work in antibiotics, and the controversy over streptomycin. Read our blog to learn more about the correlation between the flu vaccine and narcolepsy, and the evidence to support or refute the hypothesis. Read our blog to learn about the work being done to uncover how bacteria can influence the mental state. This blog discusses the blood-brain barrier, which can be compromised under certain conditions, leading to immune cell infiltration and chronic neuroinflammation. As part of their response, they secrete cytokines and chemokines, which help in the resolution of the inflammatory response.
The page also features IHC reagents for detection of neuroinflammation and a list of our neuroinflammation products.
Learn more with our podcast about some of the more popular antibody and nanobody treatments. Read about BioLegend at IMMUNOLOGY 2015™ in this blog, and we’re excited for next year's AAI Annual Meeting! Check out our blog to learn how Joseph Lister turned it all around and why he is now known as "the father of modern surgery".
In this post, we comment on the number of mechanisms that HIV, a retrovirus, has to escape the immune system. Learn about BioLegend's involvement in scholarships, talks, and interesting facts about this year's host city, New Orleans.
There's a battle brewing over the CRISPR patents with millions in funds (and profit) at stake. This allows for on-the-fly access to lab goods, and also nearly infinite customizability for lab supplies in your experiments. The fallout after the retraction of these papers just goes to show that scientific misconduct is not worth the risk. Luckily, our immune system has also “learned” how to deal with most of these pathogens, so we can eliminate most of these infections. Read through our blog to learn more about the logistics of picking out the right animal for you.
Or even my biggest mentors, who get 100x more requests for their time than I do, always seem to be free for a last minute call or lunch. Now look at each major area in your life  and the related goals (in the Goal Setting and Action workbook, we call these Areas of Attention). Give yourself time to take care of last-minute stuff that matters, and to be spontaneous with things. Read this blog to get an idea about how much it costs to run an academic research lab in the U.S. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value. If you're able to score a perfect 100%, you'll win a goodie bag filled with BioLegend prizes.
But these cells are also known for their harmful roles in neurodegenerative diseases and brain injuries, such as Alzheimer’s and Parkinson’s disease. In this post, we comment on a number of mechanisms that Salmonella has to escape the immune system. In this post, we comment on a number of mechanisms that Listeria has to escape the immune system.
Since everything takes time to do, we need to assign actual time to the things that matter most to us.



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