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Patients with abnormal cervical cytology demonstrated a higher prevalence of type-specific complement-fixing antibody to type 2 herpes simplex virus than patients with negative cervical cytology and patients with carcinoma of other body sites.
JavaScript is currently disabled, this site works much better if you enable JavaScript in your browser. The potentials and limitations of negative-selection systems based on the human herpes simplex virus thymidine kinase type-1 (HSVtk) gene, which causes sensitivity to the nucleoside analog ganciclovir, were examined in tobacco as a model system.
Case-control differences were apparent irrespective of age, socioeconomic class and marital status. The health care provider will collect the sample during an active outbreak and place it in a laboratory container. There were great differences between individual HSVtk+ transgenic plants in ganciclovir sensitivity. By contrast, case groups demonstrated a lower prevalence of subjects with type 1 specific antibody. The sample must include cells, not just fluid from the blister, because the virus is in the skin cells of the blister or ulcer. At the laboratory, the sample is placed in a special dish and watched for the growth of the herpes simplex virus, or substances related to the virus. This raises the possibility that pre-adolescent exposure to type 1 herpes simplex virus may offer some measure of protection against pre-malignant and malignant cervical pathology.
This is considered the acute phase of infection. How the Test Will Feel When the sample is collected, you may feel an uncomfortable scraping or sticky sensation. Kluwer Academic Publishers, Dordrecht, pp A3:l–19Capecchi MR (1989) Altering the genome by homologous recombination. Two independently constructed HSVtk genes showed the same variability of the phenotype in Nicotiana tabacum transformants.


Science 244:1288–1292Czako M, An G (1991) Expression of DNA coding for diphtheria toxin chain a is toxic to plant cells. This involves rubbing a sterile swab against the eye or in the throat. Why the Test is Performed The test is done to confirm herpes simplex infection. Distinct phenotypes, ranging from no regeneration through abnormal or delayed regeneration, were observed when leaf segments were placed on shoot-inducing medium supplemented with 10?6?10?3 M ganciclovir. Plant Physiol 95:687–692Czako M, Marton L (1994) The herpes simplex virus thymidine kinase gene as a conditional negative-selection-marker gene in Arabidopsis thaliana. The diagnosis is often made by physical examination (the health care provider looking at the sores), and the cultures and other tests are used to confirm that diagnosis. Normal Results A normal (negative) result means that the herpes simplex virus did not grow in the laboratory dish and the skin sample used in the test did not contain any herpes virus.
The highest HSVtk mRNA and ganciclovir sensitivity levels were observed in plants which were transformed with the pSLJ882 chimeric construct.
Plant Physiol 104:1067–1071Czako M, Jang J-C, Herr JM, Jr, Marion L (1992) Differential manifestation of seed mortality induced by seed-specific expression of the diphtheria toxin chain ‘A’ gene in Arabidopsis and tobacco. The pSLJ882 plant expression vector carried the coding sequence of HSVtk, whereas plasmid pCX305.1 carried an HSVtk construct retaining the untranslated 5 leader and viral 3 regions. Mol Gen Genet 235:33–40Depicker AG, Jacobs AM, Van Montagu MC (1988) A negative-selection scheme for tobacco protoplast-derived cells expressing the T-DNA gene 2.
The pCX305.1 transformants showed, at most, a delayed formation of shoots with thin stems and very narrow leaves. Plant Cell Rep 7:63–66Hilson P, Dewulf J, Delporte F, Installe P, Jacquemin P-M, Jacobs M, Negrutiu I (1990) Yeast RAS2 affects cell viability, mitotic division and transient gene expression in Nicotiana species. Plant Mol Biol 14:669–685Hunt AG, MacDonald MH (1989) Deletion analysis of the polyadenylation signal of a pea ribulose-1,5-bisphosphate carboxylase small-subunit gene.


A gene-dosage effect was also seen at the seedling level in the progeny of two transgenic lines.
Pl Mol Biol 13:125–138Jones JDG, Shlumukov L, Carland F, English J, Scofield SR, Bishop GJ, Harrison K (1992) Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Pl Mol Biol 18:247–258Maiti IB, Murphy JF, Shaw JG, Hunt AG (1993) Plants that express a potyvirus proteinase gene are resistant to virus infection. Cell 25:385–398Mogen BD, MacDonald MH, Graybosch R, Hunt AG (1990) Upstream sequences other than AAUAAA are required for efficient messenger RNA 3?-end formation in plants. Plant Cell 2:1261–1272Perera RJ, Linard CG, Signer ER (1993) Cytosine deaminase as a negative selective marker for Arabidopsis.
Pl Mol Biol 23:793–799Renckens S, De Greve H, Van Montagu M, Hernalsteens JP (1992) Petunia plants escape from negative selection against a transgene by silencing the foreign DNA via methylation. Mol Gen Genet 233:53–64Schardl CL, Byrd AD, Benzion G, Altschuler MA, Hildebrand DF, Hunt AG (1987) Design and construction of a versatile system for the expression of foreign genes in plants. Gene 61:1–11Stougaard J (1993) Substrate-dependent negative selection in plants using a bacterial cytosine deaminase gene.
Plant J 3:755–761Thorsness MK, Kandasamy MK, Nasrallah ME, Nasrallah JB (1991) A Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana.
Dev Biol 143:173–184Widholm JM, Kishinami I (1988) Allyl alcohol-selection for lower alcohol dehydrogenase activity in Nicotiana plumbaginifolia cultured cells.



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