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FACT: 50-60 million people are infected with genital herpes (HSV-2) today in the United States.
If antibodies against herpes simplex-2 virus are present in the sample, a typical fluorescence of the infected cells is obtained - mainly in the cytoplasm, less in the cell nuclei. As HSV-1 and HSV-2 are morphologically and immunologically closely related, cross-reactions can occur.

Differentiation may be attempted by testing a serum against both antigen substrates and comparing the titers. Identical incubation conditions and times: all tests can be combined without difficulty on one and the same microplate. Proteins from an SDS extract of HSV-1 are electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane.

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