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The presence of antibodies for HSV-2, the most common cause of genital herpes, almost always indicates genital herpes infection with the type 2 virus. HSV antibody testing can detect both viral types (HSV-1 and HSV-2) , and tests are available that can detect the early IgM antibodies as well as the IgG antibodies that remain forever in those who have been exposed.
There are several kinds of antibodies, but the two types that herpes blood tests look for are IgG and IgM.
HSV-2 also causes the herpes infection seen in babies who are delivered vaginally in women who have genital herpes. There are labs that will run tests that are not type specific, but these are essentially useless. HSV-1 usually infects the mouth, causing oral herpes, and HSV-2 usually infects the genitals, but there are a growing number of people with genital HSV-1. However, since most cases of genital herpes are caused by HSV-2, a positive result for type-2 antibodies most likely indicates genital herpes. Blood tests looking for antibodies to HSV-1 and HSV-2 may also be used to diagnose these infections. HSV-1 and HSV-2 share most of their antigenic sequences; therefore, most herpes antibodies are type-common. Genital herpes is an infection caused by either the Type 1 (HSV-1) or Type 2 (HSV-2) herpes simplex virus. Herpes simplex virus (HSV) types 1 and 2 are members of the Herpesviridae family, and produce infections that may range from mild stomatitis to disseminated and fatal disease. A number of companies offer tests that are based on relative reactivity of serum antibodies to crude preparations of HSV-1 versus HSV-2 antigens.

If antibodies against herpes simplex-2 virus are present in the sample, a typical fluorescence of the infected cells is obtained - mainly in the cytoplasm, less in the cell nuclei. A positive blood test for HSV-2 antibody usually reflects past infection of the anogenital area, although antibody tests cannot identify the site (s) of the infection (s). The presence of IgG-class antibodies to HSV types 1 or 2 indicates previous exposure, and does not necessarily indicate that HSV is the causative agent of an acute illness. Predictors of herpes simplex virus type 2 antibody positivity among persons with no history of genital herpes. For the quantitative determination of IGM Antibodies to Herpes Simplex Virus Type 2 (HSV-2) in serum.
As HSV-1 and HSV-2 are morphologically and immunologically closely related, cross-reactions can occur. However, CDC does not currently recommend routine type 2 HSV testing in someone with no symptoms suggestive of herpes infection (i. Serum herpes simplex antibodies is a blood test that looks for antibodies to the herpes simplex virus (HSV) , including HSV-1 and HSV-2. Differentiation may be attempted by testing a serum against both antigen substrates and comparing the titers. Newer type-specific assays test for antibodies to two different proteins that are associated with the herpes virus: Glycoprotein gG-1 is associated with HSV-1. Anna Wald of the University of Washington, found that people who’d had symptoms of herpes shed virus on about 20 percent of days, while people who test positive for herpes antibodies but have never had symptoms shed virus on only about 10 percent of days.
The immunofluorescent antibody technique was used to assay type-specific herpes simplex virus antibodies.

Antibody responses were studied in three groups of patients: children with primary herpetic infections, neonates with generalized type 2 infection and adults with genital herpes infections.
As a result of primary infection, the body produces antibodies to the particular type of HSV involved, preventing a subsequent infection of that type at a different site.
Identical incubation conditions and times: all tests can be combined without difficulty on one and the same microplate. But, because not everyone with herpes has symptoms, your clinician may instead use a blood test that can detect antibodies to the herpes viruses. Blood tests for herpes simplex virus type 2 (HSV-2) , the most common cause of genital herpes, are available. Proteins from an SDS extract of HSV-1 are electrophoretically separated according to molecular mass and transferred onto a nitrocellulose membrane. These blood tests can be used when someone is concerned about having been exposed to herpes, but has no visible symptoms. Transmission of type 2 genital herpes to a person who has antibodies against type 2 herpes is rare. Studies have shown that a person with genital herpes can catch a new case of genital herpes, but other studies have shown that this happens only rarely.

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