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From Chiron Biocine, Emeryville, California, and the University of Washington School of Medicine, Seattle, Washington.
Requests for Reprints: Andria Langenberg, MD, Chiron Biocine, Chiron Corporation, 4560 Horton Street, Emeryville, CA 94608.
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One hundred thirty-three patients were seropositive for only HSV-2 by Western blot, and 249 patients were HSV-1-seropositive only.

One month after publication, editors review all posted comments and select some for publication in the Letters section of the print version of Annals. Among HSV-1-seropositive patients, one immunization produced increases in gD2 and gB2 ELISA antibody titers and HSV-2-neutralizing antibody titers that were 3 to 5 times greater than those in persons with naturally acquired HSV-2 infection. Herpes simplex virus type 2 neutralizing antibodies in patients 2 weeks after the third immunization with the 30- micrograms vaccine. The dose of each glycoprotein at each injection and the number of patients receiving each dose are shown.

Among HSV-seronegative patients, frequency analysis assays showed a marked increase in the precursor frequency of gD2- and gB2-specific T cells after vaccination: T-cell responses after two immunizations were equal to the responses of HSV-2-seropositive patients and were sustained at day 180.
The geometric mean titer (GMT) to glycoproteins B2 and D2 for persons with naturally acquired HSV-2 infection are 3560 and 1209, respectively (see text).

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