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The word "probiotic" (from Greek ? create life) was first introduced in 1954 when harmful effects of antibiotics were compared with the beneficial effects of good bacteria probiotica. Modern understanding of the importance of probiotics is from the beginning of XXth century due to the Russian biologist and Nobel prize winner, the principal of the Pasteur Institute in Paris prof. In the recent years it was found out that thousands of microbial strains coexist with people. Probiotics are live microorganisms which if administered alone or by food, containing probiotics, they heal the human organism. According to the World Health Organization the total number of the probiotic bacteria with proven beneficial effects is 15. They are divided in three basic groups: lactobacilli, lactic acid cocci and bifidobacteria. Cultivation of Lactobacillus bulgaricus by the Thracians more than 7000 years ago and inventing lactic acid food is one of the three revolutions together with cultivation of crops and domestication of animals that have changed the development of mankind. Lactobacillus bulgaricus is of vegetable origin and is isolated from green plants only on the territory of Bulgaria. They stimulate the production of B-lymphocytes which identify foreign microbes and production of phagocytes which destroy them.
The lactobacilli influence on the activity of overgrowth, as they reduce the toxic metabolites and their anti-bacterial characteristics. Unlike other probiotic lactobacilli, during the lactic acid fermentation Lactobacillus bulgaricus produces D (-) lactic acid, which determines its powerful anticancerous, ray-protective and antioxidant effects. Lactobacillus bulgaricus is the largest in size probiotic bacterium and through competitive relationship inhibits the development of pathogenic (disease-causing) microbes. Having enetered in the human body, Lactobacillus bulgaricus stimulates development of the other probiotic bacteria living there such as Lactobacillus acidophilus, Lactobacillus lactis, Bifidobacterium and others. Development of the first probiotic products with Lactobacillus bulgaricus started by a team of scientists under my leadership (under the leadership of Retired Major General Prof. The products became public in 1993 and this enabled their subsequent elaboration and improvement, as well as their production and supply on the open market. We produce all our products as we keep good manufacturing practices and observe the requirements for Food Safety ISO 22000-2005.
The products do not contain preservatives, genetically manipulated microorganisms, synthetic and chemical anabolic substances. Our products are used by the Bulgarian troops during the missions in Cambodia, Kosovo, Iraq and Afghanistan, by people hurt by the earthquake in Turkey, by people working in heavy industry (cement production, asphalt bases, road construction, etc.) and by seriously ill people and children under treatment in hospitals. Daflorn Ltd produces probiotic functional foods under the trade mark Biomilk in powder, wholefood supplements under the trade marks Biomilk, Laktera and Magic Power in capsules, cosmetic products and dry face packs Mariah's face and probiotic starter cultures (yeasts), containing Lactobacillus bulgaricus and dry herbal extracts and oils. Special attention on the problem of physiological nutrition was paid in the beginning of XX century by I. Later on the concept of functional food was developed in Japan in the 80s of the last cantury. The functional probiotic food Biomilk is a new generation entirely balanced functional food. Global Scientific Researches from 2010 show that the amino acids leucine, isoleucine and valine prolong human life. Biomilk Power Energy contains an appropriate quantity of Omega 3 and Omega 6 low molecular capronic, caprylic, capric and lauric fatty acids, as well as the essential linoleic and linolenic fatty acids. Carbohydrates in Biomilk Power Energy are broken down biotechnologically to easy of digestion monosaccharides ? glucose and galactose. Atom-absorbing analyses shows that Biomilk contains great quantities of all basic macro-and microelements ? calcium, potassium, phosphorus, magnesium, iron, zinc and others. The functional probiotic food Biomilk is suitable for children, people put to severe physical or mental work, people exposed to stress and strain and the harmful influence of the environment. Immune system can be weakened when exposed to a large number of pathogens for a long time (eg.
In our clinical trials with the effect of Biomilk on Air Force pilots the obtained results are similar to those described above. Widespread and not always rational use of medicines in medical practice leads to frequent allergies and toxic-allergic reactions. Our and foreign studies show that Lactobacillus bulgaricus alters the translocation of antigens and has a favourable effect on allergies. These and other studies allow the World Health Organization in 1995 to recommend the use of Lactobacillus bulgaricus for treatment of infantile diarrhea. During the clinical study in the children's clinic of the Medical University in Sofia with our probiotic products applied to children from 6 months to 1 year, diarrhea has not been observed and the rate of the respiratory infections have decreased by 50 %. The most common cause of gastritis, stomach and duodenal ulcer and gastric cancer is Helicobacter pylori. Table 5 shows our experiment for treatment of ethyl alcohol induced ulcers in rats with different Laktera products containing Lactobacillus bulgaricus and herbal extracts.
It appears that Laktera Forte ? probiotic containing Lactobacillus bulgaricus and gin seng ? has the most powerful effect. Bowel chronic inflammations (colitis, Crohn's disease, inflammations of ileum) affect 15-20 percent of the population in the developed countries and are the major health problem.
The figure shows that after six months disbacteriosis is completely under control, colitis and gastroduodenitis have fallen down from 71 % to 4 %. The figure shows that the values of liver enzymes and ultrasound image of liver are restored after six month.
Our clinical study confirms the experimental tests with electronic microscopy, conducted in the 80 years in the Bulgarian Academy of Sciences also that treatment with Lactobacillus bulgaricus leads to stabilization of the cell membrane and restores the functions of liver cell. The probiotics Biomilk Hepanorm Forte and Laktera Rose Oil accelerate normalization of bilirubin, parameters of cholestasis and cytolysis and increase the amount of total protein.
This is confirmed by our study with 42 patients with HVC, treated without interferon, only with the probiotics Biomilk and Laktera. In the 20 century the death-rate from heart and cerebrovascular diseases increased many times. When the quantity of these components is insufficient, cholesterol remains free and is absorbed again by the human organism.
Table 6 presents our clinical study with 54 Air Force pilots from Bulgaria for 30 days, who did not change their daily regimen, their food was rich and they have taken the probiotics Biomilk and Laktera.
Effect of the lactic acid probiotic product Biomilk on fat metabolism in military pilots (Table 6).

It is obvious that regular use of the probiotics Biomilk and Laktera in combination with the main treatment of alcohol dependence leads to control of consumative and bone articular syndrome and restoration of trophic of skin and hair for a period of about 1 year.
Factors leading to cancer exist in our life and we are constantly exposed to their harmful influence. Some studies, conducted in the Agricultural department of Shinshu University in Japan, show that lactic acid bacteria have anti-mutagenic effects. The information shows that within 24 hours AF2 loses its mutagenic effect to a great extent. Lactic acid bacteria combine mutagens and carcinogens in insoluble complexes and they are removed from the body.
Similar results, demonstrating the anti-tumor effect of lactic acid bacteria, were obtained in experimental studies conducted by I. Most likely the ray-protective effect of the probiotic Biomilk is due to its immuno-stimulating effect and to limiting of the lipid peroxidation, i.e.
Development of bio radio-protectors is essential for workers in nuclear power, cancer patients undergoing chemo- and radiotherapy. According to a Report from 2005 before the Congress of USA, Lactobacillus Bulgaricus is the most important bacterium for people. It was found that some genetic changes occured first in Thracians and then in the other Europeans. They increase the production of interferon which destroys viruses and the production of antibodies acting as antidotes. This acid together with the bacteriocins and other bioactive substances, secreted by Lactobacillus bulgaricus, detoxificate the organism. Lactobacillus bulgaricus and Doderlein bacteria are part of the vaginal flora of women and determine her health and that of her children. Development of the other probiotic microorganisms is difficult and their beneficial effects are minimal without the help of Lactobacillus bulgaricus. Our products are clinically tested in leading medical institutions in Bulgaria and abroad ? MMA Sofia, Medical Universities in Varna, Sofia and Pleven, National Institute of Emergency "Pirogov", MMA in Czech Republic and Poland, Medical University of Wurzburg - Germany, together with the Medical Control of the Soviet Army and the accredited doping laboratory in Cologne. According to the Japanese Ministry of Education functional foods must have three basic functions: nutritive, sensory and healing.
It combines the healing effect of Lactobacillus bulgaricus and the nutritive and energy effects of the nutrients broken down during the lactic acid fermentation. The balanced proportions between the different fatty acids in Biomilk make it an important product for prevention of atherosclerosis that promotes the synthesis of different hormones and enzymes. The products are low lactose and can be taken by people lacking in lactase enzyme (intolerant of milk). The amino acids in the products serve as a quick transport carriers and help the full absorption of the extracts and oils by the body cells, as together with Lactobacillus bulgaricus they exalt their beneficial effects many times. Our studies show that distinct improvement of the immune resistance begins 7-10 days after the start of taking Biomilk and is at the maximum between the 15th and 20th day. One of the serious complications of medical treatment is allergic-toxic epidermal necrosis or Layel syndrome.
The use of Bulgarian yoghurt with Lactobacillus bulgaricus for treatment of diarrhea in children in Bulgaria is a century-old tradition. Frequent use of antibiotics leads to development of serious disbacteriosis and makes favourable conditions for development of fungi in intestines.
The study shows that in cases of Crohn's disease, Lactobacillus bulgaricus must be taken for a long time ? months and years and in the beginning it must be combined with the main treatment to obtain lasting beneficial effects. Figures 2 and 3 show the results from a complex treatment of addiction to alcohol and drugs in 152 patients. The figure shows that titre of antibodies against HVC in treatment only with Lactobacillus bulgaricus decreases significantly for a period of 1 year.
The complex action of the probiotic Biomilk, containing Lactobacillus bulgaricus as well as unsaturated fatty acids, amino acids and calcium, is of great importance for the indicated beneficial results.
The process of cancer formation occurs in two stages ? appearance of mutant cells as a result of damage of the cellular DNA and transformation of mutant cells into cancer cells. Products, containing Lactobacillus bulgaricus, have the most beneficial anti-mutagenic effect at the end of the 24-hour period. Widespread use of nuclear energy, radiotherapy, X-rays requires creation of radio-protective remedies. Gigov ? Department of Hematology and Oncology at the Military Medical Academy Sofia 2008 ? with 95 cancer patients deserves special attention. The new lactic acid food and the billions of beneficial (probiotic) bacteria created a gene charged with the production of the enzyme lactase which breaks down milk sugar in human body. That's why countries as China, Japan, Korea, Italy and others, offering products containing Lactobacillus bulgaricus, buy it from Bulgaria. This determines its more powerful healing and anti-toxic effect than the other probiotic bacteria. They are the next step in technological respect as compared with the other probiotic products. They can be taken in combination with each other and together with the basic medical treatment.
Pavlov ? Doctor Honoris Causa of over 120 scientific societies, academies and universities in Russia and all over the world.
With these parameters the functional food Biomilk Power Energy meets the international requirements for a standard protein food. This makes our products materially different from the other probiotic products and makes them a leader among modern probiotics.
Almost always the disease occurs with severe infectious-inflammatory complications (pneumonia, sepsis), leading to increased break-up of the body tissues. Neshev (1990) prove the beneficial effect of the Bulgarian rose oil in cases of anaphylactic shock, bronchial asthma, serotonin spasm and edema, induced experimentally in laboratory animals. Treatment with antibiotics for Helicobacter pylori is not successful because this bacterium develops an antibiotic resistance quickly and their toxic and adverse effects. Recent studies show that the best results are obtained in treatment of intestinal diseases with lactobacilli combined with the main treatment. Without Lactobacillus bulgaricus, the effect of the traditional medical treatment is temporary and recurrences are common.
According to the general opinion nutrition and taking large amounts of saturated fatty acids and animal fats causes an increase in cholesterol, especially the bad cholesterol and triglycerides.

The balanced nutritional composition of the probiotic Biomilk regulates metabolism of the human body completely.
When the immune system weakens, especially in combination with stress, the organism cannot respond adequately to the increased number of cancer cells and they begin to multiply uncontrollably. Georgieva (2007) show that the experimental animals that have taken Biomilk, normalize haematopoiesis of the bone marrow faster on the 10th day after irradiation. The patients have taken Biomilk Antioxidant in combination with the main treatment and there was statistically significant increase in the number of T-lymphocytes, thrombocytes and leukocytes and a moderate increase of hemoglobin. This new technology granted the rare privilege to people to eat throughout their lives the dairy products obtained from the milk of the domestic animals. Sc.) in 1980 as a task set by the Bulgarian government for production of special foodstuffs for the international space program "Intercosmos". Taken alone they are super concentrate of the original Bulgarian yoghurt, as only the beneficial ingredients are extracted from it biotechnologically and there is no need to consume the rest ? about 95 % with no positive effects (even lactose has harmful effects on the cardio-vascular system). Probiotics with Lactobacillus bulgaricus can be taken indefinitely and without interruption. This is the «second brain» of organism, which aims to protect from the harmful aggression of pathogens.
Photos below show the beneficial results of treatment of a case with Layel syndrome, where to the main treatment was included food therapy with 200-400 g a day functional probiotic food Biomilk.
Immediately after the antibiotic treatment, the organism is infected again which makes treatment useless. Figure 1 presents our experiment with 152 patients with gastroduodenitis, colitis and disbacteriosis for 6-month period of treatment only with Biomilk and Laktera.
It is determined both by strengthening of the immune system and by increasing the production of interferon and bacteriocins acting against viruses. Taking large amounts of these foods leads to increased excretion of bile acids through which cholesterol gets into intestines. They have many adverse and toxic effects, which are exalted in cases of taking them for a long time, which is necessary for people working in nuclear energetics and with isotopes and in cases of radiotherapy. Goranova (1992) show that Bulgarian yoghurt with Lactobacillus bulgaricus decreases the initial damages of bone marrow, accelerates restoration of haematopoiesis and decreases the death rate of experimental animals, irradiated with radioactive agents, in comparison with the control group. Metchnikoff stated that putrefactive processes develop in the human large intestine resulting in toxin production, responsible for the intestinal auto-intoxication that damages all internal organs. The produced dry probiotic foods are used by pilots from the Air Forces, Navy sailors, Bulgarian scientific expeditions to Antarctica and the Himalayas, also in cases of large-scale industrial accidents in Bulgaria.
According to Pavlov optimal nutrition must meet the enrgy and biological needs of organism. This determines the apparent effect of the products in cases of osteoporosis and low sunshine in winter and in northern regions. Fikov reported the successful treatment of infantile diarrhea with Bulgarian yoghurt, used for enemas in children's clinics in Berlin and Sofia. It was found out that Lactobacillus bulgaricus decreases the number of Helicobacter pylori in stomach on the basis of competitive elimination and by stimulation of the immune response it protects organism from infection.
Further the amino acids in the functional probiotic food Biomilk are assimilated by the liver as a building material for live and healthy cells. The cancer rate in experimental animals, fed with lactobacilli, producing L(+) lactic acid, is higher. Mechnikoff found out that this harmful influence can be eliminated by regular administration of lactobacilli. They were also used by seriously ill people and athletes training for Olympic and other world competitions. Atanasova in 36 children with leukemia and by us in 70 patients with low thrombocyte counts due to prolonged treatment with corticosteroids.
He arrived at this conclusion after he had found that the long and healthy life of Bulgarian peasants is a result from every day consumption of fermented milk and other lactic acid products made in Bulgaria.
This fact made the Thracians and the other Europeans less dependent on the sun shine and they started settling throughout the world. Complien) and according to our information Lactobacillus bulgaricus has beneficial effects in 11 directions.
Kefir does not contain Lactobacillus bulgaricus and its durability is short ? 2-7 days in refrigerator.
People do not consume energy for this and increase their energy supply for brain activity and substitution and rejuvenation of the cells. At that time the percentage of the centenarians in Bulgaria was the highest (4%), cancer rate and total sick rate were low. Solis (1993) published similar results for successful treatment of infantile diarrhea with Lactobacillus bulgaricus.
Mechnikoff wrote: "Our aging is a disease that must be treated like any other disease" and recommended every day consumption of lactic acid products. During the biotechnological process the milk proteins and casein are broken down to all 21 essential and non-essential amino acids, the milk fats are broken down to polyunsaturated Omega 3 and 6 fatty acids, the milk sugars are broken down to simple sugars. The probiotic suppresses the development of viruses (most often rotaviruses) and removes them from the intestinal tract quickly. Thracians were the first inhabitants of the present Bulgarian lands more than 7 000 years ago.
During the production lactobacilli are naturally "enveloped" with gluco-protein coat, which allows them to remain alive at room temperature for months, even years. Lactobacillus bulgaricus produces a great amount of TNFa substance which stimulates the non-specific immune response. They made the first worked gold in the world, the first script, the first grand buildings and are the basis of the development of the ancient civilizations.
This process is subject to European patent protection and is the know-how of «Daflorn» Ltd. The probiotic overcomes disbacteriosis and regulates the gastrointestinal flora during and after antibiotic treatment.

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