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admin | What Cures Bv | 13.02.2014
University of Minnesota researchers are on a mission to treat diabetes, and they’ve enlisted a few trillion microscopic helpers. In place of drugs or surgery, a team of researchers is studying how to improve diabetes patients’ insulin sensitivity by introducing trillions of beneficial bacteria into their intestines. Patients with diabetes have too much glucose in their blood, which leads to a host of serious health problems, from heart disease to obesity. The researchers’ approach to treating diabetes builds off the success of Khoruts and Sadowsky’s first “personal bioremediation” project together.
Now, with their sights set on diabetes and experts from a variety of disciplines working alongside them, Khoruts and Sadowsky are preparing to begin a clinical trial in early 2015 that focuses on patients with pre-diabetes – those who have blood sugar levels that are higher than normal but too low to be type 2 diabetes.
When the study is finished, the results will inform trials for other diseases that could benefit from this form of therapy. A method of treating incineration ash and wastewater sludge, wherein a mutual relation in using nutrients exists between the incineration ash containing heavy metal and organic wastes containing the wastewater sludge and sulfate-reducing bacteria, including the steps of burying the incineration ash together with the organic wastes under the ground, whereby the bacteria reduces the sulfates existing in the incineration ash to form sulfides, binding the formed sulfides with the heavy metal to form insoluble metal sulfides, thereby preventing the heavy metal from exuding out of the incineration ash as and eluate.
TECHNICAL FIELDThe present invention relates to a method of treating the incineration ash containing the heavy metal and the wastewater sludge, and more particularly, to a method comprising the steps of making the heavy metals existing in the incineration ashes insolulable, stabilizing the organic materials and separating from the ashes, and burying them in the conventional structure used for burying the plants.
Nucleic acid, nucleic acid for detecting dechlorination bacteria, probe, method for detecting dechlorination bacteria and method for treating earth or underground water polluted by chlorinated ethylene or chlorinated ethane - Kurita Water Industries Ltd. Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogens: Sequence of tceA and Substrate Range Characterization, by Jon K. Assessment of Indigenous Reductive Dechlorinating Potential at a TCE-Contaminated Site Using Microcosms, Polymerase Chain Reaction Analysis, and Site Data, by Donna E. ATGTTTGAAA TTCCGCATTG CTATGGTTCC AATAAGTGTT ATTATACTCA TTAATGAGAG60 ^ ^ ^ ^ ATTTACATAC TCTTCCCCGT AGGTATTAAT TCTATCTTCC TGCTGCTTAA TGTTATTTAA120 ATCCATATCA CGCTCGTTTC CCATTTTTTA CTCACGTCCC TCCCAATCTA ATTCTCCAAC180 TAACTCGCTT TGTGACTATT ATATTCTAAT TTAATAAATT TTCAATAAAA GTTTTAGTAA240 ^ ACTTTTAATA TTTTTTTTTC ATTCTTTCGT GGCACGTGCG CTTTCCAAAG TGCTATCTTC300 ^ ^ TACTTAACAA CCCTTCCC-T TAAGATAGCA T--CAAGGAG GCAGATTATG CGTA-TTGGC360 ^ ^ ^ ^^ ^ ^^ ^^ ^^ ^ TTGCGGACTA CCGCATGACA GACGCCAAAG TGCGAAAAGC TTTTTTTAA- TGAGGTATTT420 ^ TAAAAGT427 . ATGTTTGAAA TTCCGCATTG CTATGGTTCC AATAAGTGTT ATTATACTCA TTAATGAGAG60 ATATTTGTAG TTTCGCATTG CTATGGTTCC AATAAGTGTT ATTATACTCA TTAATGAGAG60 ^ ^ ^ ^ ATTTACATAC TCTTCCCCGT AGGTATTAAT TCTATCTTCC TGCTGCTTAA TGTTATTTAA120 ATTTACATAC TCTTCCCCGT AGGTATTAAT TCTATCTTCC TGCTGCTTAA TGTTATTTAA120 ATCCATATCA CGCTCGTTTC CCATTTTTTA CTCACGTCCC TCCCAATCTA ATTCTCCAAC180 ATCCATATCA CGCTCGTTTC CCATTTTTTA CTCACGTCCC TCCCAATCTA ATTCTCCAAC180 TAACTCGCTT TGTGACTATT ATATTCTAAT TTAATAAATT TTCAATAAAA GTTTTAGTAA240 TAACTCGTTT TGTGACTATT ATATTCTAAT TTAATAAATT TTCAATAAAA GTTTTAGTAA240 ^ ACTTTTAATA TTTTTTTTTC ATTCTTTCGT GGCACGTGCG CTTTCCAAAG TGCTATCTTC300 ACTTTTAATA TTGTTTTTTC ATTCTTTCGT GGCACGTGCG CTTTCCAAGG TGCTATCTTC300 ^ ^ TACTTAACAA CCCTTCCC-T TAAGATAGCA T--CAAGGAG GCAGATTATG CGTA-TTGGC360 TACTTAACAA ACCTTCCCGT TGAGTAAACA TTTCAAGGAG GCATGTTATG GATACTTGGC360 ^ ^ ^ ^^ ^ ^^ ^^ ^^ ^ TTGCGGACTA CCGCATGACA GACGCCAAAG TGCGAAAAGC TTTTTTTAA- TGAGGTATTT420 TTGCGGACTA CCGCATGACA GACGCCAAAG TGCGAAAAGC TTTTTTTAAA TGAGGTATTT420 ^ TAAAAGT427 TAAAAGT427 .
The above sequence diagram (3) shows the base sequences in the region upstream of the gene tceA of each DNA of dechlorination bacteria of two different strains by the upper and lower series thereof, respectively, in comparison of correspondence therebetween, wherein the upward arrow “^” indicates the position at which the base is different between the upper and lower series and the hyphen “-” indicates the base-defective position and wherein VC1 to VC7 indicate each the base sequence of each of SEQ ID No.
From the results of Table 1, it is seen that DNA synthesis was observed in almost all the cases for the samples of the points A, B and C where ethylenization was not observed, with exception without observed DNA synthesis of only 15 cases among 138 samples.
Researchers believe this unusual approach, conducted through a fecal microbiota transplant, could improve how the body regulates blood sugar, the central problem in diabetics. As a microbial ecologist, Sadowsky studies the complex science of how microbes interact in their environment. Products high in sugar or high-fructose corn syrup, refined grains and fast food are digested earlier on in the small intestine, leaving little food for bacteria that are located at the end of the digestive tract in the colon. The two teamed up in 2009 to take on the potentially fatal Clostridium difficile, a type of infectious bacteria that releases toxins and damages the lining of the intestines. The subjects will spend two weeks on the fixed diet before taking antibiotics to prepare them for the fecal microbiota transplant.
Lim, a professor with the U’s Department of Psychiatry, will use the imaging to help Khoruts and Sadowsky better understand how microbial balance affects communication between the brain and gut – signals that scientists are only beginning to investigate, but that could give critical insight to the eating behaviors of patients at risk for diabetes.
We may learn why diets by themselves may not be quite sufficient without the right microbes in place. The method for treatment of the incineration ash and the wastewater sludge as claimed in claim 1, wherein the said organic wastes comprise sewage sludge containing said sulfate-reducing bacteria.
A method for the treatment of incineration ash and wastewater sludge, comprising: burying the incineration ash containing heavy metal together with organic wastes containing sulfate-reducing bacteria under the ground as an accumulation, and covering them with a depth of soil so that said sulfate-reducing bacteria promote the heavy metal to precipitate in the form of dissoluble metal sulfides, whereby the heavy metal is prevented from exuding out of the incineration ash as an eluate.
The method for the treatment of the incineration ash and the wastewater sludge as claimed in claim 3, comprising two or more unit stages of the buried accumulation, each of which includes the incineration ash and the organic wastes containing the sulfate-reducing bacteria, said accumulations being constructed in a predetermined pattern of arrangement, and the depth of covering soil forming a barrier which defines a boundary between said unit stages of buried accumulation. The method for treatment of the incineration ash and the wastewater sludge as claimed in claim 3 or 4, wherein said organic wastes comprise sewage sludge containing the said sulfate-reducing bacteria.
The method for the treatment of the incineration ash and the wastewater sludge as claimed in claim 6, wherein the organic wastes comprises wastewater sludge containing said sulfate-reducing bacteria. The method for treatment of the incineration ash and the waste as claimed in claim 6, wherein a portion of the precipitated insoluble metal sulfides is extracted and only the heavy metals are subsequently recovered. And such process is based on the reasonable plan considering the mutual relations between the properties.BACKGROUND ARTThe method of disposing the solid wastes recently causes an additional problem of city life since the method depends mainly on burying them under the ground.
For treating the polluted earth or underground water, it has been proposed to introduce such dechlorination bacteria into the polluted earth or underground water. In contrast thereto, no DNA synthesis was observed for the samples of the points D, E and F where no ethylenization was detected.
The project is part of MnDRIVE (Minnesota’s Discovery, Research and InnoVation Economy), a $36 million biennial investment by the state that aims to solve grand challenges. Alexander Khoruts, a gastroenterologist at the U of M and lead principal investigator on the project, said the right balance of bacteria has the potential to improve the body’s energy metabolism, in part by enhancing insulin function. People who are diabetic or obese tend to have a less diverse microbiome – or microbial community – in their gut. Widely used across the globe, antibiotics suppress many bacteria in the gut, often disrupting the rich microbial communities that co-evolved with their human hosts for millions of years.
Then, one group will receive their own microbes back, while the other will receive microbes from selected outside donors specifically chosen as having normal blood sugar levels and good microbial diversity. The team, which also includes David Bernlohr, Ph.D, from the Department of Biochemistry, Molecular Biology and Biophysics, will conduct exploratory studies to identify the molecules involved in these signals.
Ultimately, this work should help development of new types of therapies that benefit the relationship we have with our own microbes and lead to better health. However, it is scarcely possible to secure a vast area of ground enough to bury the wastes even in the countries having the small territory in area such as Korea. It has been confirmed that such dechlorination bacteria can decompose not only chlorinated ethylene but also chlorinated ethane by means of a chlorinated ethylene-decomposing enzyme present in such dechlorination bacteria.
46, or, on the other hand, a base sequence complementary to the afore-mentioned base sequence. 56, any of base sequences each having a homology of at least 90% with respect to the base arrangement of corresponding one of the above-mentioned base sequences or any of base sequences complementary to the afore- and above-mentioned base sequences.


56, respectively, the symbol “tceA” indicates a partial base sequence found in the gene tceA and the symbol “VCr1” indicates the primer used in the Examples.As seen from the sequence diagram (3), the nucleic acids having the base sequences of SEQ ID No. One of the vials was inoculated with a bacterial suspension, from which the chlorinated ethylene-decomposing gene had been detected, in such an amount that the concentration of the chlorinated ethylene-decomposing gene will finally be 2.5?104 copies per milliliter.
As a part of MnDRIVE’s Transdisciplinary Research Program, the project will bridge multiple fields of research and bring together experts from across the U to work on the same clinical trial. In turn, they are less able to produce important short-chain fatty acids, which may regulate many functions in the body, including appetite, glucose production, metabolic rate and the immune system. In fact, on average, gut microbial communities in our society are less diverse than those found in more ancestral cultures.
However, in our society that person may turn right back to the same processed foods that are detrimental to health and do not satisfy the hungry bacteria. Khoruts and Sadowsky believe that a more diverse microbial gut community should be able to produce more short-chain fatty acids that will have a number of beneficial effects for the patients. With the balance of bacterial species thrown off, some types of microorganisms may actually become more harmful and prey on others or attack the lining of the intestine. Lisa Chow and Elizabeth Seaquist, endocrinologists from the Department of Medicine will directly measure insulin sensitivity of the participants before and after the intervention.
63, and, on the other hand, nucleic acids having base sequences complementary to the afore-mentioned nucleic acids. 56 (VC1 to VC7) hybridize in such ranges where the base sequence is different between the upper series and the lower series of the sequence diagram (2), namely, in characteristic ranges for the high performance dechlorination bacterium. Over time, the widespread use of antibiotics across the world may have caused the human gut to have less microbial diversity, creating an environment ripe for problems.
By the way, it is to be noted that, while there is a difference between the upper and lower series base sequences at the 478th and 479th bases in the sequence diagram (3), the amino acid assigned for the base sequence in this range is the same for the upper and lower series nucleic acids and, thus, no difference exists in the amino acid sequence between the decomposition enzymes expressed from them.The second group nucleic acids according to the present invention are those which are selected from the following two kinds, namely, on the one hand, nucleic acids each of which is composed of 10–50 nucleotides, preferably 15–35 nucleotides, and hybridizes with the DNA of dechlorination bacteria preferentially in the region adjoining the chlorinated ethylene-decomposing gene tceA from the 1st to the 500th base upstream of the said gene, wherein the said nucleic acids includes each a base sequence of at least 10 succeeding nucleotides, which base sequence is found in any of base sequences of SEQ ID No. 1 and 2 attached herein that the defects of the prior art, depict the amount of heavy metal contained in the incineration ash and the amount of elution, respectively. 46, any of base sequences having homologies of 90% or higher with respect to the base arrangements of the base sequences mentioned above or any of base sequences complementary to the afore- and above-mentioned base sequences. Therefore, they turned to the largely overlooked practice, first documented some 1,700 years ago, of using a fecal transplant to correct a person’s microbiome. The treatment proved spectacularly successful, able to cure approximately 98 percent of patients that failed all other standard therapies. And if entrapped by the dust collector, its concentration becomes much higher than that has not gone through the incineration. Thus, there may be recited, for example, nucleic acids having a base sequence identical with a base sequence of at least 10 succeeding bases, which is found in SEQ ID No. 50 at a position starting from any arbitrary site thereof but which is not found in any of the seven base sequences SEQ ID No. 3 shows the result of analysis for the elements of the incineration ash by use of element analyzer. 63, namely, any base sequence of a corresponding number of bases chosen arbitrarily in any of SEQ ID No.
And, the formed sulfides (S?2, HS or H2S) and the heavy metal in the incineration ash bind together due to having the attraction between them, and precipitated.Since the metal sulfides formed during the process of reducing sulfates are very stable due to having the solubility significantly lower than metal oxides, metal hydroxides or metal carboates, there is no risk of re-elution.
Most heavy metals can form the metal sulfides through the reaction with sulfates under the general anaerobic condition. For specific examples of chlorinated ethane subject to decomposition (dechlorination) by dechlorination bacteria, there may be enumerated, for example, 1,2-dichloroethane and monochloroethane.The chlorinated ethylene-decomposing gene tceA is included in the base sequence data of AF228507 registered in the GenBank of USA. In particular, even Mn, Zn and Al and so forth, which hardly precipitates as the forms of hydroxides or carboates, may react with the sulfides, to form a stable compound and to be stabilized.The heavy metals may exist in the form of salts or simple substances in the landfills of trash or wastes. In the context of this specification, “gene tceA” means not only that of the base sequence entered in the base sequence data of AF225807 but also one which has a homology of at least 90% of the gene arrangement of this gene.The second group nucleic acids according to the present invention can be used for detecting high performance dechlorination bacteria, especially those exhibiting high activity of decomposing vinyl chloride, selectively in an easy manner with specifically high accuracy by carrying out a PCR (polymerase chain reaction) using the second group nucleic acid as the primer or by performing hybridization. The heavy metals existing as the dissolved form in the landfills of trash 4or wastes may move as the ionic state, and such pathway for the heavy metal to be insolubilized may be called to comprise the steps of adsorption and precipitation.
The adsorbed heavy metals can move again when the adsorption reaches the saturation point or the adsorbents are decomposed. Therefore, it may be mentioned that the final insolubilization of heavy metals in the landfill is mainly carried out by the precipitation.The metal sulfides formed by binding sulfides and heavy metals may be the stable compounds in the solid form, unless being affected by the external condition such as aerobic environment or low pH value. These labelling materials can be introduced into the nucleic acid by known techniques.By using the dechlorination bacteria detecting probe according to the present invention, a dechlorination bacterium can be detected at a high accuracy in an easy manner, by inspecting a sample of bacterium by hybridizing it with the labelled probe, followed by detection of the labelling material in the dechlorination bacterium having the hybridized labelled probe by an adequate technique.
The reason is that since the solubility of metal sulfides is extremely low, the heavy metal scarcely eluded from the metal sulfides.
Thus, presence of a dechlorination bacterium in an inspection sample can be judged, when DNA of an expected size is synthesized on examination by PCR using any one of the nucleic acids according to the present invention as the primer and a nucleic acid prepared from the sample to be inspected as the template. Therefore, once the metal sulfides formed, they are not re-eluded.The solubility products of metal sulfides at 25° C. Especially, when the examination is carried out using the second group nucleic acid according to the present invention, high performance dechlorination bacteria can be detected selectively.PCR can be performed in a manner known per se, wherein use of a commercial kit for PCR may be permissible.
For performing PCR, two primers, namely, an upper primer and a lower primer, are usually incorporated, wherein it is permissible to use the nucleic acids according to the present invention as either one or both of these primers. By performing a plurality of PCRs using a plurality of different nucleic acids as the primer, the reliability of detection can be increased.The method for detecting dechlorination bacteria according to the present invention is based on the use of the labelled probe for detecting dechlorination bacteria according to the present invention. Thus, the method is performed in such a way that the labelled probe for detecting dechlorination bacteria according to the present invention is brought into contact with a sample containing bacteria to be inspected for the presence of dechlorination bacteria or with the nucleic acid(s) prepared from such sample to cause an RNA- or DNA-hybridization, whereupon the detection of the bacteria is effected by using the label as indicator. As a result, it is expected that the possible contamination of soil and underground water due to the elution of heavy metal in the treatment of incineration ash can significantly be decreased.The present invention uses the sulfate-reducing bacteria in order to solidify the harmful heavy metal contained in the incineration ash into the precipitates in the stable form of metal sulfides.
In this regard, since like the most of organism, the sulfate-reducing bacteria also use the energy, which has been obtained during the anabolism such as the decomposition of organic compounds, a certain organic material should also be provided in order to utilize the metabolism of the sulfate-reducing bacteria. Especially, when the detection is carried out using a probe labelled with a second group nucleic acid according to the present invention, high performance dechlorination bacteria can be detected selectively.Detection of the dechlorination bacteria after the hybridization can be realized by a known technique in accordance with each specific labelling material employed.
Since the sulfate-reducing bacteria use the various organic compounds as the carbon source, they are in the advantageous position relative to the other microorganisms in an aspect of competition for securing the organic compounds.Since the sulfate-reducing bacteria is an anaerobic or at least almost anaerobic microorganism, an anaerobic or at least almost anaerobic condition should be provided for the sulfate-reducing bacteria to grow in the said environment.


Thus, the detection may be realized for the case where a radioactive element is used for the label, by observing the radioactive ray by a known technique. When a fluorescent substance is used as the label, the detection can be attained by observing the fluorescent light by a known practice.
Furthermore, although not provided to Table 2, the amount of contents as well as that of eluent of heavy metal is very low in the sewage sludge.
When an enzyme is used as the label, the detection can be attained by determining the enzymatic activity.
When a chemical substance is used as the label, the detection can be effected by analyzing the substance by a known method.
However, no sulfate-reducing bacterium exists in the incineration ash.An alternative example of the organic waste containing the sulfate-reducing bacteria includes the soil from the paddy field. When an antigen or an antibody is used as the label, the detection can be realized by carrying out an antigen-antibody reaction using an antibody or an antigen capable of reacting specifically with the labelled antigen or antibody and analyzing the resulting reaction product by known techniques.If no dechlorination bacterium is detected by the method for detecting dechlorination bacteria according to the present invention using the first group nucleic acids according to the present invention, it is judged that dechlorination bacterium are absent. By performing the method for detecting dechlorination bacteria according to the present invention using the second group nucleic acid and utilizing the thereby obtained high performance bacterial dechlorination additive, the polluted environment can efficiently be treated (decontaminated).The bacterial dechlorination additive to be introduced into the polluted environment may be any of those detected (collected) at any place. After reducing by use of CO2 gas and sealing, the bacteria in the container were cultured at 30° C. Furthermore, it is possible to increase the ratio of removing the heavy metals in the incineration ash by the anaerobic condition formed by the sulfate-reducing bacteria as well as the other kinds of bacteria existing in the organic wastes and decomposing the organic compounds. When sufficient treatment of the polluted earth or underground water is not attainable by a single dose of the bacterial dechlorination additive, it may be possible to perform several repeats of doses of the bacterial dechlorination additive. 3 shows the result of analysis for the elements of the incineration ash by use of element analyzer.FIG. 4 shows the schematic pathway of the sulfates to be reduced into the sulfides by the sulfate-reducing bacteria.FIG. 49)) were designed from the base sequence data of AF228507 registered in the GenBank in USA, wherein an adjoining region of the chlorinated ethylene-decomposing gene was utilized.
The base sequence data of AF228507 include the chlorinated ethylene-decomposing gene tceA registered.By PCR synthesis, clones which have high homologies with respect to the base arrangement of the chlorinated ethylene-decomposing gene tceA were obtained from four underground waters, of which base sequences were determined. 6 schematically shows the process of Example 1 for carrying out the method for treatment of the incineration ash according to the invention.FIGS. The base sequence of the chlorinated ethylene-decomposing gene was the same for all the four underground water samples and had a homology of 96% with respect to the reported base arrangement of the gene tceA. 7a to 7d provide the explanation of the types of the unit stacks buried in the landfill.FIGS. The determined entire base sequence of the chlorinated ethylene-decomposing gene is recited in SEQ ID No. 6 schematically shows the process of Example 1 for carrying out the method for treatment of the incineration ash according to the invention.As shown in the drawings, below portion of the filling-up structure of landfill is provided a waterproofing barrier(61) and a drainage net system of leachate(62), and such units of the filling-up structure(63) are arranged to comprise the repeated layers.
Each unit of the filling-up structure(63) should be covered by the layer of covering soil(64).As seen from FIGS. 7a to 7d, the units of the filling-up structure(63) may be constructed in the various manner of accumulating or mixing the incineration ash and sewage sludge.For instance, as seen from FIG. 7a, after filling up the sewage sludge (A), the incineration ash (B) is filed up on the first layer, and the layer of covering soil (64) may be covered; as seen from FIG. To this mixture were then added 250 ?l of 10% SDS solution and the resulting mixture was held thermostatically at 65° C. 7b, after filling up the mixed trash (A+B) comprising the sewage sludge (A) and the incineration ash (B), the layer of covering soil (64) may be covered; as seen from FIG.
7c, after filling up the sewage sludge (A), the city trash (C) and the incineration ash (B) are sequentially filed up on the first layer, and the layer of covering soil (64) may be covered, or as seen from FIG. Then, the treated mixture was centrifugated at 8000?g for 10 minutes at room temperature and the resulting supernatant was collected.
7d, after filling up the mixed trash (A+B+C) comprising the sewage sludge (A), the incineration ash (B) and the city trash (C), the layer of covering soil (64) may be covered.The filling-up structure of landfill having the predetermined size may be formed by the repeated work for constructing the units of filling-up structure (63). The supernatant was treated with chloroform to remove contamination proteins, whereupon the separated upper aqueous layer was isolated and processed by addition of an equivolume of isopropanol, allowed to stand for 60 minutes at room temperature and centrifugation at 8000?g for 20 minutes at room temperature to precipitate the DNA.
On the upper end of the filling-up structure in landfill, the final layer of covering soil (66) is laid. Therefore, the heavy metal in the incineration ash is solidified to stable metal sulfides in the units of the filling-up structure (63). For the primer pair, each 20 pmol of each pair among the 46 sets of primer pairs given in Table 1 were used. For other components of the reaction liquor composition, the guides of the manual attached to the PCR kit were followed. 8a, the leachate (81) from the region of the incineration ash and the leachate (82) from the region of the sewage sludge in the landfill are collected in one reaction plant (85). 8b, in the mixture (86) in the reaction plant (85), the heavy metals existing in the leachate from the incineration ash may be precipitated by the action of sulfate-reducing bacteria in the sewage sludge. 8c, the precipitated heavy metal (87) and the remaining reaction product (88) are separately drained out and treated.
In this stage, since the precipitated heavy metal (87) may be recovered through the additional apparatus for recovering the heavy metal, an advantage of the possible re-use of resources may be accomplished. Furthermore, since the reaction production may contain an amount of the sulfate-reducing bacteria adopted to the anaerobic condition, it may be reused in the process for treatment of the incineration ash.INDUSTRIAL APPLICABILITYThe present invention enables the conversion of heavy metals in the incineration ash into the stable metal sulfides in the form of insolubilized precipitates, thereby significantly reducing the possibility of contaminating the surrounding soils and underground water caused by the elution of heavy metals during the treatment of incineration ash.
Furthermore, in case that an organic waste such as the sewage sludge which contains the sulfate-reducing bacteria as the sulfate-reducing microorganism source, a synergic effects for treating the heavy metals existing in the sludge may also be expected. Moreover, another kinds of bacteria existing in the waste sludge besides the sulfate-reducing bacteria may amplify the effect for removing the heavy metals from the incineration ash.



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