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24.01.2014
UTR, which stands for Untranslated Region, refers to either of two sections on each side of a coding sequence on a strand of mRNA. The three prime untranslated region (3' UTR) is a particular section of messenger RNA (mRNA). Binding sites for proteins, that may affect the mRNA's stability or location in the cell, like SECIS elements (which direct the ribosome to translate the codon UGA as selenocysteines rather than as a stop codon), or AU rich elements (AREs), stretches consisting of mainly adenine and uridine nucleotides (which can either stabilize or destabilize the mRNA depending on the protein bound to it).
Binding sites for proteins, that may effect the mRNA's stability or translation, for example iron responsive elements, which occur in the 5' UTRs (and 3' UTRs) of a small number of eukaryotic mRNAs that regulate gene expression in response to iron. The LightSwitch Luciferase Assay System is a unique collection of over 30,000 precloned regulatory elements from the human genome. Measure RNA stability, translation efficiency and the functional impact of miRNAs on a gene-by-gene basis. A constitutive promoter drives the production of a hybrid luciferase - human 3'UTR construct.
If it is found on the 5' side, it is called the 5' UTR, or if it is found on the 3' side, it is called the 3' UTR.


This marks the site of cleavage of the transcript approximately 30 base pairs past the signal, followed by the addition of several hundred adenine residues (poly-A tail). It starts at the +1 position (where transcription begins) and ends just before the start codon (usually AUG) of the coding region. The mRNA also contains regions that are not translated: in eukaryotes this includes the 5' untranslated region, 3' untranslated region, 5' cap and poly-A tail.
Some viruses and cellular genes have unusual long structured 5' UTRs which may have roles in gene expression.
Gene expression is detected by measuring the luminescence of a novel, highly sensitive luciferase, RenSP.
And even though the road’s covered by a thick canopy of trees, you occasionally get these breathtaking views from high atop cliffs that overlook Lake Michigan. In eukaryotes the cap, 5' untranslated region, 3' untranslated region, and polyA tail (see diagram). It usually contains a ribosome binding site (RBS), in bacteria also known as the Shine-Delgarno sequence (AGGAGGU).


Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection in most studies. The 5' UTR may be a hundred or more nucleotides long, and the 3' UTR may be even longer (up to several kilobases in length) (Molecular Cell Biology, 5th edition, Lodish et al. Hover over the SHOW INFO button and choose a season.What was the name of that restaurant that featured that guy who made food?
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