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This journal is a member of and subscribes to the principles of the Committee on Publication Ethics. Recombinant vectors are herpes simplex virus type 1 (HSV-1) particles carrying an engineered HSV-1 genome.
Flow cytometric analysis for endothelial marker CD31 (a) and hematopoietic marker CD45 (b) on human breast cancer cells. Recombinant vectors can be (1) attenuated, in which case they carry a mutation in a gene affecting virulence (white circle) and generally a reporter gene (in this case luciferase, white arrow) to facilitate following the spread of the virus, or (2) defective, in which case they carry a deletion in at least one gene encoding an essential function (grey circle). Defective vectors generally carry a transgene of interest (red arrow) and a reporter gene (in this case green fluorescent protein (GFP), green arrow) to identify the infected cells.

Immunofluorescent staining of vimentin, fibronectin and cytokeratin in primary cancer cells (c) and foreskin-derived fibroblasts (d).
Amplicon vectors (3) are HSV-1 particles carrying a head-to-tail concatemer of a DNA derived from the amplicon plasmid, instead of the virus genome. We found that foreskin-derived fibroblasts strongly expressed fibroblastic markers vimentin and fibronectin, but these cells were negative for cytokeratin.
In addition, they carry one HSV-1 origin of DNA replication (oriS) and one packaging signal ('a') to allow amplification and packaging of the plasmid into HSV-1 particles. In contrast, human primary breast cancer cells strongly expressed cytokeratin, whereas these cells were negative for vimentin or fibronectin.

Owing to the absence of genes encoding viral proteins, the amplicon plasmid can be amplified and packaged only in the presence of an HSV-1 helper genome.

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  1. Krasavcik 10.05.2016 at 10:14:29
    Completely different sickness before i found that herpes by coming into the skin or mucous.
  2. Smert_Nik 10.05.2016 at 22:22:57
    Herpes encephalitis is an irritation of the mind search of industry companions to provide are.
  3. NIKO_375 10.05.2016 at 20:16:48
    Herpes zoster and its spreading.