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When checked, Shutterstock's safe search screens restricted content and excludes it from your search results. Science, Technology and Medicine open access publisher.Publish, read and share novel research. Propolis: Alternative Medicine for the Treatment of Oral Microbial DiseasesVagner Rodrigues Santos1[1] Universidade Federal de Minas Gerais, Faculty of Dentistry, Department of Clinical, Pathology and Surgery, Laboratory of Microbiology and Biomaterials, Brazil1.
PRGF®-Endoret® Technology+ LLLT Therapy What is itBTI's research team has developed the therapeutic use of plasma rich in growth factors (PRGF®-Endoret®).
They are proteins that we all have and which carry out an essential function in complex processes of tissue repair and regeneration. Its clinical efficiency in the stimulation of tissue healing and regeneration has been described in a large number of international articles. In none of the treatments carried out over the years have any undesired effects been observed.
It is a pioneer (Patent no EP1066838) in the development of specific protocols for tissue regeneration.
It is a versatile system that makes it possible to create different formulations that adapt to the characteristics of each application.
The latest advances in medicine are increasingly leading to the adoption of new minimally invasive therapies and procedures. An alternative to the healing of wounds that have difficulty scarring and an innovative treatment that achieves a very natural facial rejuvenation.
At BTI we are carrying out important research and making an enormous clinical effort to develop new formulations and protocols for the application of PRGF®-Endoret® with the aim of improving the treatment of a wide range of clinical situations. Minimum Inhibitory Concentration (MIC) of propolis ethanolic extract and control obtained for each strain tested.
Clinical aspects of oral candidosis in patients with Total Removable Dental Prothesis (TRDP). Mean scores of Severity Gengival Index (DP) and percent reduction between periods (Pereira et al., 2011) [29]. Clinical aspects of patients with oral candidiasis from Clinic of Semiology and Pathology of Dentistry School UFMG participating in this study and Results of in vivo patients treatment of oral candidiasis with 20% Brazilian green ethanol propolis extract (EPE) and Nystatin (Nys). IntroductionBees are arthropods of Hymenoptera order and are classified into two groups based on their type of life: solitary and social life.
It is a technology that allows the use of the organism's own resources with extraordinary results in many pathologies, effectively regenerating tissues without side effects and notably reducing the recovery period of fractures, muscular and tendinous injuries, and surgical interventions. The therapeutic application of growth factors stimulates and accelerates the processes of tissue healing and regeneration. In this respect, PRGF®-Endoret® has opened new paths and therapeutic approaches thanks to its accessibility and reparative capacity. Propolis is produced by bees that live socially, from the harvesting of products derived from plants and used to seal and protect the hive against intruders and natural phenomena [1]. B) Confirmation of insertion loss and presence of periodontal pockets with periodontal probe. Propolis term derives from the Greek Pro, "opposite, the entry" and polis, "city or community" [2,3]. D) Clinical aspect of the periodontium after treatment with gel containing propolis (Cairo do Amaral et al. Characteristically, it is a lipophilic material, hard and brittle when cold, but soft, flexible and very sticky when warm. It has characteristic odor and shows adhesive properties of oils and interact strongly with skin proteins [6]. Some factors, such as the botanical origin of propolis and its time of collection can influence the chemical composition of this resinous material [9].

The color of propolis varies from yellowish green to dark brown, depending on location - savannah, tropical forests, desert, coastal and mountainous regions - where it is produced. Propolis is used by bees to protect against the entry of microorganisms, fungi and bacteria in the hive, and as a sealing material for preventing the entry of light and moisture inside.
It is also used to line the comb, to allow the deposition of eggs by the queen, and to embalm small dead animals (beetles and insects) that usually bees could not take into the hive, preventing its putrefaction.3,5,7]. Interest in the pharmacological action of natural products has grown and found significant popular acceptance. Among these products, propolis has been highlighted due to its applicability in the food industry and cosmetics, to be used as the active ingredient in several products, among which include toothpastes and skin lotions [13].
Regarding the ethnobotanical aspect, propolis is one of the few "natural remedies" that continue to be used for a long period by different civilizations [14]. Propolis is widely used in popular medicine, especially in communities with inadequate public health conditions[15]. Significant decrease in H?O?-induced DNA damage in cultures treated with propolis demonstrated antioxidant activity of phenolic components found in propolis may contribute to reduce the DNA damage induced by H?O? [16].2. The Egyptians, who knew very well the anti-putrefactive properties of propolis, used it for embalming [17].
It is the only technique that holds the obligatory health certifications required for each one of the following applications: Healing of venous ulcers Consolidation of bone fractures and grafts Fixation of prostheses in the hip, knee, etc.
It was recognized for its medicinal properties by Greek and Roman physicians, such as Aristotle, Dioscorides, Pliny and Galen. Oral implantology Injuries and breakages of ligaments, muscles and tendons Treatment of skin burns, injuries and lesions Regeneration of aged skin It is a versatile system that makes it possible to create different formulations that adapt to the characteristics of each application. The drug was used as an antiseptic and healing in the treatment of wounds and as a mouthwash, and its use in the Middle Ages perpetuated among Arab doctors [2]. Also, it was widely used in the form of ointment and cream in the treatment of wounds in battle field, because of their healing effect.
Applications: The latest advances in medicine are increasingly leading to the adoption of new minimally invasive therapies and procedures.
This healing propolis property known as "Balm of Gilead," is also mentioned in the Holy Bible [18]. From the pharmacological point of view, propolis has been used as solid; in an ointment based on vaseline, lanolin, olive oil or butter, and in the form of alcoholic extract and hydroalcoholic solution. In the 1980s and 1990s, a great number of publications occurred worldwide, highlighting Japan in number of published papers followed by Brazil and Bulgaria [6]. In Dentistry, there are studies investigating the pharmacological activity of propolissome situations, such as gingivitis, periodontitis, oral ulcers, pulp mummification in dogs' teeth and dental plaque and caries in rats [19]. Also, it has been used in dressings of pre and post-surgical treatment, oral candididosis, oral herpes virouses and oral hygiene. There was also the investigation of antiseptic and healing properties of propolis in subjects admitted to various hospitals and the results were extremely positive [20]. Thus, this natural product revealed great interest for the treatment of oral diseases [21]. Internationally, the first licensed commercial product containing propolis was registered in Romania in 1965.
In the 1980s, commercial licenses were predominant in the former USSR and satellite countries. Currently, 43% of commercial licenses are Japanese origin and 6.2% of them are products for dental treatment. In Japan, the scientific productivity reported for propolis increased 660% between the 1980 and 1990 [22]. The global interest in propolis research increased considerably in relation to its various biological properties [23-27].

Another incentive for conducting research on propolis is a high value on the international market, mainly in Japan, where a bottle of ethanol extract is sold at prices ten times higher than that prevailing in Brazil.
Brazil is considered the third largest producer of propolis in the world, behind Russia and China only. Japan’s interest for the Brazilian propolis is due to its therapeutic and organoleptic properties, and also the presence of minor amounts of heavy metals and other environmental pollutants [28,29]. In the last thirty years, various studies and scientific research were performed to clarify the medicinal properties attributed to propolis [30,31]. The main types of botanical origin are South (three), Northeast (six) and Southeast (twelve), and they were reported as resins from Populus sp., Hyptis divaricata and Baccharis dracunculifolia (Figure 5), respectively.
An attempt to classify propolis produced in Brazil according to botanical origin and chemical composition [32] has recognized 12 different types. It was suggested that Hyptis divaricata is the resin source of northeastern propolis, Baccharis dracunculifolia of southeastern propolis and poplar (Populus nigra) of southern propolis. The different compounds present in Brazilian propolis were identified and quantified using high performance liquid chromatography (HPLC) technique. Established the process of separation by liquid chromatography, capable of identifying the major components of propolis samples (primary marker). Through the technique of HPLC and quantification of compounds identified by it, it was established a classification for Brazilian propolis based on the presence of markers (Table 1 and Table 2). The main feature of this classification relates to the speed in which this product bee can reach the market, from the field to the pharmaceutical and cosmetic industries, encouraging the use of these typing for the manufacture of their medicines and cosmetics, with established quality control, since all of these markers were separated in a concentration range types. Another important factor is that the classification will be possible to manufacture pharmaceuticals, cosmetics and oral hygiene products knowing the propolis type used and the quantities of bioactive components, features never reported before in publications and patents on propolis [33]. These plants are a group of woody perennial shrubs, which are dioecious with male and female inflorescences appearing on separate plants. Of the 30 different species of Baccharis, Baccharis dracunculifolia is the dominant source of propolis in southeastern Brazil (Sao Paulo State and ??Minas Gerais State), where most of propolis based products sold are produced [34].
Recently, it was founded a red type of propolis in hives located in mangrove areas in the Northeast. It was observed that bees collect exudate from the surface of red Dalbergia ecastophyllum (Linnaeus, Taubert) (Figure 6). Analysis and comparision of plant exudates and propolis samples demonstrate that the chromatographic profiles are exactly the same as the one found for D. The best way to find the plant origin of propolis would be by comparing the chemical composition of propolis with the alleged plant origin [36]. Chemical compositionTable 1 and Table 2 show the chemical markers constituents of green and red Brazilian propolis, respectively, while Table 3 shows the chemical composition of various types of world propolis.
The highest concentration of phenolic compounds was obtained using solvents with lower concentrations of ethanol and higher concentrations of crude propolis, but the highest concentration of flavonoids in the extract was obtained with higher concentrations of ethanol in the solvent [11]. Among the chemicals constituents, we can include waxes, resins, balsams, oils and ether, pollen and organic material.
The proportion of these substances varies and depends on the place and period of collection [5,37]. The collected propolis in a bee hive, also known as crude propolis, in its basic composition, contains about 50% of plant resins, 30% of beeswax, 10% essential oils, 5% pollen, 5% debris of wood and earth [7,14,6]. Propolis also contains various organic acids, considerable amount of minerals (including, manganese, zinc, calcium, phosphorus, copper), vitamins B1, B2, B6, C and E, acids (nicotinic acid and pantothenic acid) and aminoacids [5,7,11,38].

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