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The HBV DNA kit employs the salting-out method to extract DNA from blood serum or plasma to serve as template.
The addition of internal control and dUTP-UNG enzyme system prevents false negatives and false positives due to carry-over contamination from previous PCR products. The procedure is simple and fast, has high sensitivity, and give direct and accurate results. No cross-reactivity with Herpes simplex virus type 1 and 2, Varicella zoster virus, Epstein-Barr virus, Cytomegalovirus, Herpes simplex virus type 6, HIV, Hepatitis A virus, Hepatitis C virus, Yellow fever virus, Human T-cell leukemia virus type 1 and 2, Coxsackievirus B3, and Dengue fever virus. HBV-IC is set up as a positive internal control which monitors the whole experiment starting from nucleic acid extraction. UNG enzyme + dUTP setup is used to effectively prevent contamination of PCR products from previous reactions, which prevents false positive results and increase its reliability for clinical diagnosis. Common treatment drugs such as IFN? (2a, 2b and 1b), PEG-IFN? (2aand 2b), lamivudine, adefovir dipivoxil, entecavir, and telbivudine do not affect detection results.

Competent to test samples with high viscosity protein, high bilirubin, and high triglycerides. Compatible with PCR instruments from ABI, Roche, Bio-rad etc, allowing for broader applications. High throughput tests suitable for detection and analysis with 96-well plate or 384-well plate instruments. Quantifying HBV viral load in serum of patients with chronic Hepatitis B infection can provide a supporting evidence for the diagnosis of chronic Hepatitis B, and differentiate between patients with chronic Hepatitis B and those with inactive HBsAg.
HBV DNA results (normal or elevated ALT) is used as treatment indication for patients with HBV-related decompensated chirrosis or liver transplant.
Post-treatment follow-up monitoring for chronic Hepatitis B patients; HBV DNA levels are tested once a month for the first 3 months to prevent early relapse, followed by testing every 3-6 months.
It uses real-time fluorescence-based quantitative PCR technology to quantify HBV DNA through measuring changes in the fluorescence signals.

Using fluorescence based real-time PCR technique, the samples and a series of standard solutions with known concentrations of the target gene are simultaneously amplified and detected under the same cycling conditions.
These samples were simultaneously tested with Supbio’s HBV DNA kit and another similar product that has better quality and is nationally recognized. The amount of target gene in the samples can be quantified using a carefully generated standard curve. Results for HBV-IC reaction can then be used to determine if there are PCR inhibitors in the samples and prevent false negative results.

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