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SDS-PAGE was performed followed by an in-gel trypsin digest in preparation for high resolution mass spectrometry. In comparison with a previous proteomic analysis,4 the Bell group identified 67 out of the predicted 82 proteins, with at least 2 unique peptides discovered.

These totals included HSV1 structural proteins consisting of capsid proteins (91%) and tegument proteins (96%) but only 79% consisting of envelope proteins.

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