Sensitive cells like stem cells, primary cells, neural cells, and immune cells are much less resilient
Changing culture conditions affect them comparatively more than immortalized cell lines. Unlike established cell lines that have been growing in culture for decades, slow-growing cell types or those that have only been passaged a few times have not had the opportunity to adapt (mutate) to artificial, two-dimensional in vitro culture conditions.
These fragile cell types generally require more exacting and labor-intensive effort to successfully culture [1]. Also, any microbial contamination is comparatively more costly if these cultures have to be discarded and the work repeated.
The Cell Locker System is ideal for sensitive cells including stem cells, primary cells, neural cells, lymphocytes, and more.
1. Gibco™ Pluripotent stem cell guidebook (2017). Thermo Fisher Scientific COL31555 0417.