iBind Flex Western Device

iBind Western Device

The last step in the western workflow is detection. In this step, primary antibodies specific to the protein of interest bind to the protein on the membrane. Secondary antibodies conjugated to horseradish peroxidase (HRP) or alkaline phosphatase (AP) are then added, and they bind to the primary antibody to enable visualization of the protein bound to the membrane. Our automated western blot processing systems allow you to set up your blot for processing in minutes, then walk away. No more shakers, trays, timers, or babysitting your western blots.

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Automated western blot processing

Automated blot processing workflow

The Invitrogen iBind Flex Western Device offers the same automated blot processing technology as the original iBind Western System, but comes with interchangeable wells. These interchangeable wells provide blot-format flexibility and enable the use of different primary and secondary antibody conditions in a single experiment.

The Invitrogen iBind Western Device is an automated western blot processing platform. Simply load primary antibody, secondary antibody, and wash solutions, and then walk away. In less than 3 hours, the blot is ready for final detection.