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Manual
iBind Western Device
Phospho-EGFR
For more information about the target proteins and antibodies used, go to thermofisher.com/ibindflex
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The iBind Western System enables excellent western blot results with less primary antibody.
Excellent western blot results with vertically cut strips and fluorescence detection. Comparison of mini blots processed manually (probing and washing steps performed in a tray) vs. with the iBind Flex Western Device. Blots were produced by separating samples on Bolt 4–12%, 10-well gels with MES SDS running buffer, rapid-dry transfer to nitrocellulose membrane using the iBlot 2 system, and then cutting each into three-lane strips. Final imaging was performed using a fluorescence imaging instrument.
Samples and lanes were as follows:
Blots processed with iBind and iBind Flex devices
iBind Flex
Manual