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iBind Western Device

Phospho-EGFR

For more information about the target proteins and antibodies used, go to thermofisher.com/ibindflex

The iBind Western System enables excellent western blot results with less primary antibody.

Excellent western blot results with vertically cut strips and fluorescence detection. Comparison of mini blots processed manually (probing and washing steps performed in a tray) vs. with the iBind Flex Western Device. Blots were produced by separating samples on Bolt 4–12%, 10-well gels with MES SDS running buffer, rapid-dry transfer to nitrocellulose membrane using the iBlot 2 system, and then cutting each into three-lane strips. Final imaging was performed using a fluorescence imaging instrument.

 

Samples and lanes were as follows:

  • Lane 1: Thermo Scientific PageRuler Prestained NIR Protein Ladder (3 μL)

  • Strip 1: Phosphorylated Akt cell extract (15 μg, 7.5 μg, 3.75 μg) and Elk-1 fusion protein (150 ng, 75 ng, 37.5 ng)

  • Strip 2: HeLa cell extract (30 μg, 15 μg, 7.5 μg)

  • Strip 3: Phosphorylated Akt cell extract (15 μg, 7.5 μg, 3.75 μg)

  • Strip 4: Elk-1 fusion protein (150 ng, 75 ng, 37.5 ng)

  • Strip 5: HeLa cell extract (30 μg, 15 μg, 7.5 μg)

  • Strip 6: HeLa cell extract (30 μg, 15 μg, 7.5 μg)

 

Blots processed with iBind and iBind Flex devices

iBind Flex

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