As with other components in a western blotting system, there are many chemiluminescent substrate choices available. The appropriate selection of a substrate depends on the detection level (sensitivity) required, the target protein's abundance, and the sample's availability.
Select the optimal chemiluminescent substrate for your western blot application from our five substrates.
Chemiluminescent substrates
Choose the appropriate chemiluminescent substrate for western blot detection
For data above: STAT3 detection in HeLa cell lysate (lane 1: 20 μg total protein; lanes 2–6: two-fold serially diluted) was performed using Thermo Scientific HRP chemiluminescent substrates. The blots were developed using Invitrogen Anti-STAT3 Antibody (Cat. No. MA1-13042) and Goat Anti-Mouse IgG Secondary Antibody, HRP conjugate (Cat. No. 31430). Images were captured using the Thermo Scientific myECL Imager (Cat. No. 62236).