Sarcopenia is the decline of muscle mass and strength with age. Evidence suggests that oxidative stress and molecular inflammation play important roles in age-related muscle atrophy. The two factors may interfere with the balance between protein synthesis and breakdown, cause mitochondrial dysfunction, and induce apoptosis. Sarcopenia, inflammation and oxidative stress is highly prevalent in hemodialysis patients and may contribute to mortality. The copy number of mitochondrial DNA (mtDNA) is affected by oxidative stress in blood circulation. This study aimed to test whether mtDNA copy number correlates with oxidative stress and some uremic toxins in nondiabetic hemodialysis(HD) patients. 200 nondiabetic hemodialysis patients and 50 healthy subjects will be enrolled. This study will be performed to investigate quantitative changes in mtDNA occur in HD patients with and without sarcopenia. Copy number of mtDNA in leukocyte DNA is determined by real-time polymerase chain reaction in HD patients and 50 age- and sex-matched control subjects. In addition, correlation of the alterations of albumin redox status, 8-isoprostane, plasma IL-6 ,LBP and TNF-a and as well as various uremic toxins will be performed.
Description: The copy number of the mtDNA and the nDNA is calculated using the threshold cycle number (Ct) and intrapolating from the standard curve. The ratio of the copy number of mtDNA to the copy number of nDNA is measurement of mtDNA content.
Measure: Determination of mtDNA/nDNA Ratio as a measure of mtDNA Content Time: 1 yearsDescription: ndoxyl sulfate (IS) and para-cresol (p-cresol) belong to the group of protein-bound uremic toxins that are poorly cleared by dialysis and are associated with poor clinical outcomes.the group of protein-bound uremic toxins that are poorly cleared by dialysis and are associated with poor clinical outcomes.
Measure: Plasma uremic toxins analysis Time: 1 yearsDescription: Total 8-iso-PGF2-alpha concentrations in plasma were measured by a specific enzyme immunoassay (EIA) kit
Measure: Total of 8-iso-PGF2-alpha Analysis Time: 1 yearsDescription: The IL-6 was determined from serum samples and controls using standardized enzymelinked immunosorbent assay (ELISA) methods
Measure: Analysis of biomarkers of IL-6 Time: 1 yearsDescription: The TNF-α was determined from serum samples and controls using standardized enzymelinked immunosorbent assay (ELISA) methods
Measure: Analysis of biomarkers of TNF-α Time: 1 yearsDescription: The LBP was determined from serum samples and controls using standardized enzymelinked immunosorbent assay (ELISA) methods
Measure: Analysis of biomarkers of Liposaccharide-binding protein (LBP) Time: 1 yearsDescription: the fractions of HMA, HNA-1, and HNA-2 to total HSA were measured using HPLC
Measure: Measurement of the Albumin Redox State Time: 1 yearsCase-Crossover
There is one SNP
We quantified the analytes by using the analyte to standard peak area ratio on a Agilent 1100 High Performance Fluorescence detector G1321A. --- G1321A ---