RATIONALE: Studying samples of blood from patients with cancer in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at DNA variations in the RASSF1A gene in young patients with Wilms' tumor.
Name: molecular genetic technique
Type: Genetic
Name: polymerase chain reaction
Type: Genetic
Name: polymorphism analysis
Type: GeneticCase Control
There are 2 SNPs
Presence of the A133S polymorphism of the RASSF1A tumor suppressor gene in germline DNAs of children with Wilms' tumor. --- A133S ---
Inheritance pattern of the A133S polymorphism in the parents of children who carry this polymorphism. --- A133S ---
Differences in age at diagnosis, stage, histology, site of primary tumor, and outcome between patients with vs without the A133S polymorphism. --- A133S ---
DISEASE CHARACTERISTICS: - Diagnosis of Wilms' tumor OR - Control participants matched for race, sex, and age - No prior or concurrent history of cancer OR - Parents of children involved in the study PATIENT CHARACTERISTICS: - Not specified PRIOR CONCURRENT THERAPY: - Not specified DISEASE CHARACTERISTICS: - Diagnosis of Wilms' tumor OR - Control participants matched for race, sex, and age - No prior or concurrent history of cancer OR - Parents of children involved in the study PATIENT CHARACTERISTICS: - Not specified PRIOR CONCURRENT THERAPY: - Not specified Kidney Cancer Kidney Neoplasms Wilms Tumor OBJECTIVES: Primary - Determine the presence of the A133S polymorphism in the RASSF1A tumor suppressor gene by screening germline DNAs of children with Wilms' tumor. --- A133S ---
- Determine the inheritance pattern of the A133S polymorphism by evaluating the parents of children who carry this polymorphism. --- A133S ---
Secondary - Determine the differences in age at diagnosis, stage, histology, site of primary tumor, and outcome, between patients with vs without the A133S polymorphism. --- A133S ---
DNA is also analyzed from parents of patients with the A133S polymorphism. --- A133S ---
Whether the S131F RASSF1A variant is a true polymorphism. --- S131F ---
- Determine whether the S131F RASSF1A variant is a true polymorphism in these patients. --- S131F ---