Three laboratories will participate in the study. Each laboratory will analyze the same samples by different methodologies according to the flow indicated in figure 1. This design will allow comparing the agreement performance of different methods available for T790M identification in circulating-free DNA isolated from peripheral blood.
Name: Tirosin Kinase Inhibitors
Description: To evaluate the agreement performance of different methodologies available in Spain for T790M identification in circulating-free DNA isolated from blood collected at the time of progression on a first or second generation TKI
Measure: Assess the agreement between qualitative methodologies Time: At 12 months from the first inclusionDescription: To compare the cost of the different methodologies
Measure: Cost of the different methodologies Time: At 12 months from the first inclusionDescription: To estimate the specificity and sensitivity of each cfDNA method.
Measure: Specificity and sensitivity of each cfDNA method Time: At 12 months from the first inclusionDescription: To compare turnaround time of the different methodologies
Measure: Turnaround time of different methodologies Time: At 12 months from the first inclusionDescription: To compare the ease of use of the different methodologies
Measure: Ease of use of different methodologies Time: At 12 months from the first inclusionCohort
There is one SNP
"T790M Mutation Testing in Blood by Different Methodologies"_RING Project. --- T790M ---
T790M Mutation Testing in Blood by Different Methodologies Three laboratories will participate in the study. --- T790M ---
This design will allow comparing the agreement performance of different methods available for T790M identification in circulating-free DNA isolated from peripheral blood. --- T790M ---
To evaluate the agreement performance of different methodologies available in Spain for T790M identification in circulating-free DNA isolated from blood collected at the time of progression on a first or second generation TKI. --- T790M ---
Circulating free DNA from peripheral blood sample is an adequate source for T790M resistance mutation testing. --- T790M ---